Simultaneous Determination of Four Tetracycline Antibiotics Residues in Chicken by Solid Phase Extraction Coupled with High Performance Liquid Chromatography


  •  Ming Zhao    
  •  Yiting Li    
  •  Zhiqiang Ji    
  •  Xiuping Dou    
  •  Chaoling Zhang    
  •  Xiaohan Li    
  •  Shufang Liu    
  •  Li Zhao    
  •  Na Liu    

Abstract

Objective: To establish a simple and rapid method for simultaneous determination of Tetracycline, Oxytetracycline, Chlortetracycline and Doxycycline in chicken. Methods: After optimized sample pretreatment and chromatographic conditions, tetracycline antibiotics in spiked chicken were extracted by ultrasonic extraction with Na2EDTA-Mcllvaine buffer solution (pH=4.0), purified and enriched by HLB solid phase extraction column, separated by Shim-pack GIST C18 column (4.6×150 mm, 3 μm), methanol-acetonitrile-oxalic acid (1:2:7, V: V: V) as the mobile phase, the flow rate was 0.9 mL/min, the UV detection wavelength was 350 nm, and the peak area was quantified by external standard method. Results: The linearity of the four tetracycline antibiotics was good in the range of 0.05-1 mg/kg (r>0.997), and the detection limits were 2.53 μg/kg ~ 5.94 μg/kg according to the S/N ratio (S/N) of 3. The relative standard deviations (RSD, n=6) of precision were 1.51%-4.80%. The recoveries were from 76.81% to 96.34%. Conclusion: The method has good selectivity, high accuracy and reproducibility, and is suitable for simultaneous determination of four tetracycline antibiotics in chicken.



This work is licensed under a Creative Commons Attribution 4.0 License.
  • ISSN(Print): 1927-0887
  • ISSN(Online): 1927-0895
  • Started: 2012
  • Frequency: quarterly

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