Thermal Stabilization of HEWL by Adsorption on Biochar

  •  Hidetaka Noritomi    
  •  Ryotaro Kai    
  •  Nobuyuki Endo    
  •  Satoru Kato    
  •  Katsumi Uchiyama    


We have found that the heat stress tolerance of hen egg white lysozyme (HEWL) is markedly enhanced by the adsorption of HEWL on bamboo charcoal powder (BCP), which is a kind of biochar. HEWL was firmly adsorbed on BCP even at high temperatures. The secondary structure of HEWL was altered to some extent by the adsorption of HEWL on BCP. The remaining activity of BCP-adsorbed HEWL exhibited more than 20% after the incubation for 30 min at 900C although that of free one was hardly observed. Moreover, the half-life of BCP-adsorbed HEWL was 13 min at 900C while that of free one was 4 min.

This work is licensed under a Creative Commons Attribution 4.0 License.
  • ISSN(Print): 1927-0585
  • ISSN(Online): 1927-0593
  • Started: 2012
  • Frequency: quarterly

Journal Metrics

Google-based Impact Factor (2017): 5.94
h-index (January 2018): 13
i10-index (January 2018): 20
h5-index (January 2018): 12
h5-median (January 2018): 26

Learn more