Molecular Characterization and Genetic Diversity of Yellow Passion Fruit Based on RAPD Markers
- Angélica Vieira Sousa Campos
- José Ricardo Peixoto
- Fábio Gelape Faleiro
- Michelle Souza Vilela
- Márcio de Carvalho Pires
Abstract
Molecular genetic variability studies are essential to complement the agronomic characterization of yellow passion fruit genotypes (Passiflora edulis Sims). Therefore, this study aimed at evaluating the genetic diversity of 24 genotypes of yellow passion fruit obtained from a research program developed by the University of Brasilia and Embrapa Cerrados, using RAPD molecular markers. RAPD markers were obtained from eight decamer primers and converted into a matrix of binary data, from which genetic dissimilarities among genotypes were estimated, and clustering analysis was performed. A total of 54 RAPD markers were obtained, with 6.8 bands per primer on average. From this total, 46 (85.2%) RAPD markers were polymorphic. The OPD10 primer presented the highest number of polymorphic bands. The high percentage of polymorphic markers evidenced the existence of genetic variability among genotypes. Nei’s genetic distance between genotypes ranged from 0.043 to 0.451. Clustering resulted in the formation of at least five groups of similarity.
- Full Text: PDF
- DOI:10.5539/jas.v11n3p575
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