Polyacrylamide electrophoresis gel for studying multilocus enzyme uses the relative electrophoretic mobilities of intracellular enzymes to characterize and differentiate organisms by generating an electromorph type. There are many articles for enzyme electrophoresis by using starch gel but there is lacuna by using polyacrylamide gel especially in shrimps. This article presents the chemical conditions for some enzyme that may be useful, a guide to the successful practice of the electrophoretic technique, by using polyacrylamide gel in shrimps.