Abstract

Plant parasitic nematode, Heterodera glycines (Soybean cyst nematode, SCN) is the major pathogen of Glycine max (soybean). This pathogen is widely distributed, seriously disserving, and has a wide approach for spreading. Huipizhi Heidou (ZDD2315), a germplasm resource of soybean originated in China, has an excellent resistance to SCN. In this paper, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were employed to separate the differentially expressed proteins from soybean induced by SCN. The F₄ separated populations from the cross between the resistant Huipizhi Heidou and the susceptible Liaodou 15 were used as test materials by bulked segregant analysis. The 2-DE gels analysis revealed 367 protein spots from the resistant samples and 372 protein spots from the sensitive samples. Among those protein spots, 23 protein spots from the resistant samples and 4 protein spots from the sensitive samples were differentially expressed, and then were selected for peptide mass fingerprinting (PMF) and sequencing assay by MS. Of the 27 differentially expressed proteins, 16 protein spots were identified by MALDI-TOF-MS and 11 were not identified due to low scores. Further analysis showed that the majority of these 16 proteins were involved in defense, energy and metabolism, suggesting that they might be related to the soybean resistance to SCN.

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