Biological Control of Leaf Spot and Growth Promotion of Eucalyptus Plants by Trichoderma spp.

  •  Magno Rodrigues Carvalho Filho    
  •  Irene Martins    
  •  Gustavo Henrique Silva Peixoto    
  •  Paulo Henrique Pereira Costa Muniz    
  •  Daniel Diego Costa Carvalho    
  •  Sueli Corrêa Marques de Mello    


This study aimed to evaluate the potential of twelve Trichoderma isolates on eucalyptus leaf spot control induced by Cylindrocladium scoparium and determine the sporulation capacity of the isolates on two substrates (parboiled rice and millet) for selection of potential biocontrol and growth promoter agents, in eucalypt seedlings. In the in vitro experiments, volatile and non-volatile metabolites tests showed different levels of inhibition of the pathogen mycelial growth. In tests on detached leaves, it was observed suppression of disease symptoms with all Trichoderma strains in leaves inoculated with CEN494 isolate of the pathogen, whereas with isolate CEN517 did not found the same efficiency. Sporulation of Trichoderma was higher using parboiled rice as the substrate, compared to millet. In general terms, it was obtained 3.38 × 109 and 2.84 × 109 conidia/g for the two substrates, respectively. Some of these Trichoderma isolates may be used in development of biofungicides for biocontrol of leaf spot, especially CEN262, due to its suppressive effect on C. scoparium, high sporulation, growth promotion capacity and endophytic colonization in eucalyptus seedlings.

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