Abstract

The objective of this study is to screen four species of marine actinomycetes: Gordonia (SPTG111), Rhodococcus sp (BP33), Gordonia sp (BP5), Brevibacterium antarcterium (SPTG45). These bacteria were grown in seven different media, namely Braine Heart Infusion broth (BHIB), Nutrient broth, Marine broth, Trypticasein Soy Broth, Tryptic Soy Broth, Luria Bertani broth, and Lauryl Sulfate Broth. Brine heart infusion broth (BHIB) was used to growth bacteria for the antibacterial activity test. Crude extract of Gordonia tearrae was tested for antimicrobial activity against Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA), Bacillus cereus, Enterococcus faecalis, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Candida albicans using agar diffusion method. The minimal inhibitory concentration (MIC) of G. tearrae crude extract was determined by the microbroth dilution method. Ethyl acetate extract of this isolate showed significant antibacterial activity against Gram negative bacteria E. coli (20 mm), Pseudomonas aeruginosa (9 mm), Salmonella typhi (12 mm) and Gram positive bacteria E. faecalis (7 mm), Bacillus cereus (21 mm), and MRSA (30 mm), and yeast C. albicans (21 mm). The MIC value of antimicrobial activity ranged from 25 to 6.3 µg/ml.

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