Abstract

Functional male sterility in tomato (Solanum lycopersicum L.), controlled by the ps-2 and ps genes can be utilized in the production of F₁ hybrid tomato seed. Two novel cleaved amplified polymorphic sequence (CAPS) markers linked to the ps-2 and ps genes were found in tomato. The C4-30 and C2-21 markers were developed based on the conserved ortholog set II (COSII) sequences C2_At3g20020 and C2_At1g65900 located on tomato chromosomes 4 and 2, respectively. The HinfI-derived PCR restriction product C4-30₈₀ was applicable in the detection of functional male sterile plants. In case of the C2-21 marker, a polymorphism was revealed after digestion of the amplicon with restriction enzyme MboI. Specificity of the DNA markers identified was verified by scoring the tomato parental lines, F₂ progeny and F₁ hybrids, in which maternal lines possessed the ps or ps-2 gene. C4-30 and C2-21 can be used as the diagnostic tools in tomato breeding programs and in F₁ hybrid seed production.

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