The Influence of Sludge on the Lolium Antioxidant Enzymes Activity and Malondialdehyde Content


  •  Jiao Chen    
  •  Yuanxiang Yang    
  •  Xuemei Zhu    
  •  Lei Wang    
  •  Shengnan Li    
  •  Pengcheng Dai    

Abstract

Using the soil of abandoned lead-zinc tailings and heavy metal enrichment plants (Lolium) as materials, through the outdoor pot experiment, study the influence of sludge on antioxidant enzymes (CAT, SOD and POD) activity and malondialdehyde (MDA) content. The results showed that: CAT activity increased with the increase of sludge concentration, increased first and then decreased, at concentration (278g/kg) to reach the highest: aerial parts 4.224 [u/(gFW.min)], roots1.532 [u/(gFW.min)]; SOD and POD activity increased with increasing sludge concentration trends, high concentrations (417g/kg) reached the highest, concentration of aerial parts and root SOD were 0.2134 [W/(h.gFW)] and 0.1376 [W/(h.gFW)]. Aerial parts of Lolium MDA content increased with sludge concentration increased, while roots was manifested in the opposite trend.The study showed that high concentrations (417g/kg) sludge can significantly improve antioxidant enzyme activities of Lolium, reduce cell damage and increase their resistance to lead-zinc stress.



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