Abstract

Detection of phytoplasma classified in phylogenetic group 16SrI, ‘Ca. Phytoplasma asteris’, in the plants of interspecific genotypes of Brassica oleracea x Brassica napus and their back-cross toward B. oleracea (IW7.7xB1) is reported in Poland. The presence of phytoplasmas in leaves of plants showing failure of flower bud formation but not in the asymptomatic hybrids was demonstrated by polymerase chain reaction assay employing phytoplasma universal P1/P7 primer pair. Amplified cDNA fragments of two phytoplasma isolates were sequenced. The nucleotide sequences of the Brassica hybrids phytoplasma isolates showed more than 98,5 % similarity with the corresponding fragments of sequence of phytoplasmas belonging to group 16SrI, ‘Ca. Phytoplasma asteris’.

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