Abstract

The procurement of inoculum for bioassays with target spot, a significant disease in cotton and soybeans, caused by Corynespora cassiicola can be hindered by the low production of conidia on artificial culture media. The study aimed to determine whether mechanical stress on the mycelium and exposure to ultraviolet-C (UV-C) radiation for varying durations could enhance conidial production in C. cassiicola isolates. Eight isolates from cotton and soy were used, grown in V8 juice medium. After five days of incubation in a climate-controlled chamber, each isolate either underwent mycelium scraping or remained unscraped and was subsequently exposed to UV-C radiation for either 1.0, 1.5 or 2.0 min, in comparison to a control group with zero UV exposure time. Following these procedures, conidial suspensions from each isolate were obtained and quantified (conidia per mL) using a Neubauer chamber The ISO 3S and ISO 4S isolates were found to produce more conidia than the other isolates, regardless of whether they were subjected to mycelium scraping or exposure to UV-C radiation. For most isolates, exposure to UV-C radiation for 1.0-1.5 min led to increased conidium production. Generally, it was not feasible to discern differences in conidial production with respect to the mycelium scraping process. Nevertheless, exposure to UV-C radiation for 1.0 min can be used to induce conidium production in C. cassiicola isolates.

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