Influence of Sulphate Nutrition on Growth Performance and Antioxidant Enzymes Activities of Spirulina platensis
- Pierre Fils Rodrigue Magwell
- Emile Minyaka
- Oscar Wamba Fotsop
- Marlyse Solange Leng
- Léopold Gustave Lehman
Abstract
The growth of Spirulina platensis is dependent on culture conditions. This study has established adequate conditions for the quality and quantity production of S. platensis. The effect of sulphate salts nutrition on growth performance and biochemical status of S. platensis was assessed in vitro. Prior to culture, the Paracas strain of S. platensis from SAGRIC pond was analysed in different magnesium sulphate (MgSO4; 0.08, 0.16, 0.32, 0.64 and 1.28 g/L), potassium sulphate (K2SO4; 0.08, 0.16, 0.32, 0.64 and 1.28 g/L) and MgSO4/K2SO4 (0.16/0.00, 0.08/0.08, 0.04/0.12, 0.02/0.14 and 0.01/0.15 g/L) concentrations. Culture media pH, total dissolved solids (TDS) and conductivity rate were monitored. Microscopic analysis revealed sulphate salt concentrations influenced the number of whorls and filaments of S. platensis. K2SO4 (1.28 g/L) produced the highest number of whorls and filaments. Moreover, pH level fluctuated by sulphate treatments. K2SO4 (1.28 g/L) had a pH level of 8.77±0.01 (day 5 of culture incubation). TDS and conductivity rate, protein and cysteine contents increased with culture age and K2SO4 concentration in a culture medium. Conversely, negative correlations between protein and cysteine contents were observed, and sugar content decreased. Sulphate salt type and concentrations affected polyphenol oxidase (PPO) and peroxidase (POD) activities. MgSO4/K2SO4 (0.02/0.14 g/L) displayed the best PPO and POD activities. Both enzymes appeared to be negatively correlated to the decreasing sugar content. These results indicate growth performances and biochemical status of S. platensis are significantly improved with the adequate supplementation of sulphate salts (MgSO4 and K2SO4) in culture media.
- Full Text:
PDF
- DOI:10.5539/jas.v13n10p115
Journal Metrics
The data was calculated based on Google Scholar Citations.
- h-index (December 2021): 67
- i10-index (December 2021): 700
- h5-index (December 2021): 20
- h5-median (December 2021): 28
Index
- ACNP
- AGRICOLA
- AGRIS
- BASE (Bielefeld Academic Search Engine)
- CAB Abstracts
- CiteFactor
- CNKI Scholar
- CrossRef
- DESY Publication Database
- DTU Findit (DTU Library)
- EBSCOhost
- Elektronische Zeitschriftenbibliothek (EZB)
- Excellence in Research for Australia (ERA)
- FindIt@Bham (UoB Library)
- Genamics JournalSeek
- Google Scholar
- HOLLIS (Harvard Library)
- Index Copernicus
- Infotrieve (Copyright Clearance Center)
- Jisc Library Hub Discover
- JournalTOCs
- Library of Congress
- LIVIVO (ZB MED)
- LOCKSS
- LSE Library
- Max Planck Institutes
- Mendeley
- MIAR
- Mir@bel
- Norwegian Centre for Research Data (NSD)
- OAJI
- Open J-Gate
- OskiCat (UCB Library)
- PKP Open Archives Harvester
- Publons
- Qualis/CAPES
- ROAD
- Scilit
- SHERPA/RoMEO
- Southwest-German Union Catalogue
- Standard Periodical Directory
- Stanford Libraries
- StarPlus (UoS Library)
- Technische Informationsbibliothek (TIB)
- Trove
- UCR Library
- Ulrich's
- UniCat
- Universe Digital Library
- University Library (USask)
- WorldCat
- WorldWideScience
- WRLC Catalog
- Zeitschriften daten bank (ZDB)
Contact
- Anne BrownEditorial Assistant
- jas@ccsenet.org