Abstract

Technological production processes of organic dyes soluble in water, as well as the processes for their application in
textile industries, may heavily pollute natural waters, particularly from the point of view of their pronounced colored
wastewaters. Reactive dyes are prominent among numerous groups of water-soluble dyes. The bioflocculant was
effective in flocculating a kind of reactive soluble dyes (Cibacron yellow FN_2R) in aqueous solution. A bioflocculant–
producing bacterium were isolated from wastewater and sediments of Close Drainage Systems (CDS) located at the Prai
industrial area.. Compared with conventional chemical flocculants, bioflocculants are biodegradable and nontoxic, and
produce no secondary pollution. Sphingomonas paucimobilis was found to produce a bioflocculant with high
flocculating activity for Kaolin suspension and water-soluble dyes. The best temperature flocculation performance was
35°C and shaking speed of 160 rpm. The highest flocculating efficiency achieved for Kaolin suspension was 98.4% at
35°C after 48 hours cultivation. Various culture temperatures were tested between 2 hours in order to investigate their
effect on the bioflocculant production when the culture temperature was 35°C which the flocculating activity of
Sphingomonas paucimobilis was up to 98.4%. It was found that, flocculating rate depends on time and temperatures.
Determination flocculating activity was shown Sphingomonas paucimobilis is biodegradable and increase in number of
bacteria during the time will confirm that. This study was conducted to biologically treat wastewater discharged from
the textile industry using sequencing batch reactor (SBR) technology biological flocculation on COD removal and
effects of solids detention times and MLVSS on EPS production.

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