In vitro and in vivo Evaluation of Hemocompatibility of Silk Fibroin Based Artificial Vascular Grafts

  •  Derya Aytemiz    
  •  Yu Suzuki    
  •  Tomoko Shindo    
  •  Toshiki Saotome    
  •  Ryou Tanaka    
  •  Tetsuo Asakura    


Artificial vascular grafts with low thrombogenicity are generally required to avoid blood platelet adhesion and to minimize intimal hyperplasia, thus retaining vascular patency. In this study, we aimed to determine the acute and subacute hemocompatibility of silk fibroin (SF) grafts by in vitro and in vivo evaluation. Blood contact reaction with SF grafts was examined by thrombin-anti-thrombin III complex (TAT) formation, platelet activation level by beta-thromboglobulin (beta-TG), complement system response (C3a and SC5b-9), platelet and fibrin deposition and compared with commercially available polyethylene terephthalate (PET) artificial grafts in vitro. The biocompatibility and coagulation-inducing effect of coating materials were evaluated by in vivo implantation in rats. Two weeks after implantation, SF grafts showed low subacute coagulation. All blood parameters evaluated for animals implanted with SF-coated grafts showed almost the same values as those for sham-operated animals. Our results support the suggestion that SF will be a suitable material for vascular regeneration in future.

This work is licensed under a Creative Commons Attribution 4.0 License.
  • ISSN(Print): 1916-9698
  • ISSN(Online): 1916-9701
  • Started: 2009
  • Frequency: semiannual

Journal Metrics

h-index (December 2022): 32

i10-index (December 2022): 145

h5-index (December 2022): N/A

h5-median(December 2022): N/A

( The data was calculated based on Google Scholar Citations. Click Here to Learn More. )