Preparation of Low Molecular Weight Chitosan by Complex Enzymes Hydrolysis

  •  Huafei Xie    
  •  Zhengyu Jia    
  •  Jinhui Huang    
  •  Cuirong Zhang    


Through the determination of viscosity average molecular weight, enzyme activity, hydrolysis ratio and reducing sugar concentration, the optimal technological conditions of complex enzymes composed of commercial ?-amylase, cellulase and pectinase were investigated. Results indicated that complex enzymes could result in low molecular weight chitosan ranged from 1000 to 4000 after enzymatic degradation for 2 h under the condition of zymolyte ratio 1:5(m/m), pH 5.3 and temperature 56?, and the structure of those products failed to vary obviously by FTIR analysis.

This work is licensed under a Creative Commons Attribution 4.0 License.
  • ISSN(Print): 1916-9698
  • ISSN(Online): 1916-9701
  • Started: 2009
  • Frequency: semiannual

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