Lignocellulosic Processing with Acid Pretreatment and Enzymatic Hydrolysis for Improving the Acquisition of Sugar Fermentation

  •  Nuniek Hendrianie    
  •  Sri Juliastuti    
  •  Moch. Izati Iwani    
  •  Affrida Eka    


Banana peels is a waste which has not been widely used, so that the relatively long time the existence of such waste brings its own problems such as pollution. Banana peels contain high enough lignocellulosic and can be degraded into simpler forms. The Lignin content of banana peels needs to be removed / destroyed structure. The purpose of this study was to determine the effect of the addition ratio of Trichoderma reseei and Aspergillus niger on hydrolysis liquefaction and saccharification time in stage hydrolysis to glucose produced with the highest glucose parameters. In this study, the method was used to degrade lignin by using sulfuric acid (2%). Afterward, the cellulose content was changed in liquefaction process. Hydrolysis liquefaction performed using Aspergillus niger and Trichoderma reesei with a mixture ratio of 1: 0, 0: 1, 1: 1 and 1: 2. Furthermore, substrate hydrolysis liquefaction saccharification going through the process of hydrolysis to increase glucose levels were formed. Hydrolysis liquefaction aims to change the content of starch in the banana skin into glucose using Saccharomyces cerevisiae as the addition of 20% (v / v). Hydrolysis process of liquefaction is done by varying the sampling time on day - 3, 6, and 9. In this study the hydrolysis of liquefaction of banana peels use a mixture of Trichoderma reesei and Aspergillus niger 2:1 at a temperature of 50oC, pH 5, and 64 h for resulted in glucose with the best content was 0.52%. In addition, the results showed that the hydrolysis saccharification with the addition of Saccharomyces cerevisiae glucose levels were highest on day 3 with a temperature of 30oC and pH 5, resulting in glucose content to 1.63%.

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