Abstract

[Objective] To isolate and screen strains that could yield both amylase and protease in the early stage of biogas slurry, determine the vitality of amylase and protease and improve the rates of biodegradation and gas yield .[Method] Use selective medium for early screening, and then apply the modified Yoo method and national professional standards concerning determination of protease activity to determine the vitalities of amylase and protease.[Result] Isolate and screen one strain that can yield both amylase and protease in the early stage of biogas slurry. The vitality of amylase and protease was 102.61U/mL and 92.93U/mL, respectively.

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