Biochemical Changes in Relation to Brown Leaf Spot (Drechslera oryzae) Resistance in Different Rice Genotypes
- K. Bisen
- Virendra Kumar
- Kishan Lal
- Rakesh Kumar
- Nand Kumar
AbstractBrown leaf spot resistance in twelve rice genotypes viz IRRON M2 201, IRRON M2 202, IRRON M2 203, IRRON M2 204, IRRON M2 205, IRRON M2 302, IRRON M2 303, IRRON M2 304, IRRON M2 401, IRRON M2 402 and IRRON M2 501 were tested that the genotypes were showing 5.10 -20.16, 6.15-30.31 and 9.30-38.71 per cent disease severity at vegetative, panicle initiation and milk dough stages of plant growth, respectively which has also indicated that stages of plant growth also give variable response to disease. The mechanism of resistance revealed that the higher amount of total phenol and soluble protein in rice leaves resulted lower disease incidence. The rice genotype IRRON M2 205 is having maximum amount of soluble protein, representing 22.46, 21.94 and 21.27mg/gm and total phenol of 1.79, 1.68 and 1.61mg/gm of fresh leave at vegetative, panicle initiation and milk dough stage, respectively. Correlation between total phenol and soluble protein with disease severity at different growth stages shows that there was a negative correlation in all the rice genotypes. The rice genotype namely IRRON M2 205 was showing correlation value (r) -0.3310, -0.3415 and -0.3510 at vegetative stage, panicle initiation stage and milk dough stage, respectively. Banding pattern of protein resolving in SDS-PAGE showed that the maximum number of protein bands (13) was found in genotype IRRON M2 205. The protein banding patterns might be the responsible factors for resistance in rice against brown leaf spot pathogen.
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