Studies on Genetic Diversity of Selected Population of Hybrid Scallop Chlamys farreri(♀)× Patinopecten yessoensis(♂) by Microsatellites Markers


  •  Biao Wu    
  •  Aiguo Yang    
  •  Ningning Cheng    
  •  Xiujun Sun    
  •  Zhihong Liu    
  •  Liqing Zhou    

Abstract

The growth superiority of hybrid scallop Chlamys farreri (♀) × Patinopecten yessoensis (♂), as the following successive generation selection have been reported. However, the data about the genetic diversity in those population remains unexplored. In this study, the genetic structure analysis of F1, F2 and F3 were conducted by PCR with 10 Simple Sequence Repeats (SSR) primers. It showed that a total of 68 alleles were detected, and the number of alleles per locus ranged from 3 to 11, Polymorphism Information Content (PIC) per locus ranged from 0.4729 to 0.8429. And, the average observed heterozygosity (Ho) of the three populations were 0.6100, 0.6975 and 0.7750, while the average expected heterozygosity (He) were 0.7607, 0.7751 and 0.7379 respectively. Fst values among the three populations were also low (Fst<0.05) which suggested low genetic differentiation between each two populations. In all, those data indicated the genetic structure challenge caused by hybridization and selection, supplying a new angle to understand artificial selective breeding.



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