Abstract

The optimized experimental parameters of degradation obtained with a four-factor at three-level orthogonal array
experimental design L18(3^6) were temperature, rotation speed, inoculum size and liquid level by Corynebacterium
variabile in the shaking bottle as 33.C, 22h after shaking at the same temperature and 120 rpm for 14h, 4%, 80mL in
150mL triangle bottle, respectively. Among four factors, liquid level is paramount.

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