Abstract

Sequencing msh locus of the bundle forming pilus (Bfp) of Aeromonas veronii bv sobria and characterization of mshA
promoter region for further research. A direct genomic sequencing and genomic walking were performed to sequence
Bfp. PCR, PCR screen, restriction reaction, and agarose gel electrophoresis were attempted to construct a reporter gene
gusA contained plasmid. We extended the sequence by 3.4 kb. Some encoded proteins and a promoter between mshB
gene and mshA gene was detected. Work with promoter region did not succeed. The sequence got so far still contained
some errors, though 15 encoded proteins and a promoter were found. But the reason that the promoter region project did
not work was unknown.

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