In Silicon Cloning and Analysis of a LACS Gene from Glycine Max (L.)

  •  Lili Yu    
  •  Xiaoli Tan    
  •  Weiwei Yuan    
  •  Fuge Zhu    


Long chain acyl-coenzyme A synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters, and play important
roles in the biosynthesis and degradation of lipids. In this study, a Glycine max(L.) LACS gene, designated as GmLACS,
has been isolated through in silicon cloning. The gene is 2,219 bp with an open reading frame (ORF) of 1,989 bp, which
encodes a LACS with 662 amino acid residues, with the isoelectric point of 6.42 and the calculated molecular mass of
65.6 kDa. Sequence analysis showed that GmLACS possessed typical domains of LACSs. Real-time quantitative PCR
data analysis suggested that GmLACS was hightly expressed in leaves and young pods.

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