Abstract

Peroxiredoxins are thiol-specific antioxidants that protect cells from oxidative damage and have proliferative and
anti-apoptotic activity. We investigated the effect of serum and phorbol ester treatment on expression of Prdx1 and Prdx6
in H2.35 cells, and the possible role of Sp1 on Prdx6 induction. Serum stimulation induced a 30% increase in Prdx1
mRNA and a three-fold increase in Prdx6 mRNA. We showed a similar effect of phorbol ester treatment, which led to a
30% increase in Prdx1 mRNA, and over a two-fold increase in Prdx6 expression. Analysis of the Prdx6 proximal
promoter sequence revealed four consensus Sp1 sites. Inhibition of Sp1 with mithramycin A blocked Prdx6 induction by
TPA and inhibited the serum-induced transcriptional activity of the Prdx6 proximal promoter. These data suggest an
important role for Prdx6 in the cellular response to serum and TPA, and implicate Sp1 as a possible mediator of Prdx6
regulation.

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