Cloning and Analysis of DNA Sequence of Gene CylA of Enterococci Inducing Sheeps Encephalitis


  •  Guijun Ma    
  •  Sujuan Han    
  •  Genqiang Yan    
  •  Xia Zhou    

Abstract

A DNA fragment was amplified from the chromosomal DNA of Eerococci Inducing Sheeps Encephalitis by PCR and
then was cloned into pMD19-T Vector. The cloned fragment was sequenced. Comparing the DNA sequence of the
amplified fragment with the published sequence of gene CylA showed that they were 99.3% homologous.


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