Quantitative Real-Time PCR Detection of Airborne Staphylococcus aureus in Hospital Indoor Atmosphere

Zhitang Lu, Jing Wang, Yating Zhang


Staphylococcus aureus is a classical type of gram-positive bacteria. It is attached to skin, mucous membranes, especially pharynx nasalis, as an opportunistic pathogen which can cause a wide variety of infectious diseases. With increasing use of antibiotic the resistance of Staphylococcus aureus is increased. And more and more methicillin-resistant Staphylococcus aureus which caused seriously disease emerged recent years. So a sensitive, rapid, and specific method was developed using real-time PCR to detect the number of Staphylococcus aureus survived in atmosphere of a specific ward. In this study thirteen samples were collected in all during the second quarter of 2011.

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DOI: https://doi.org/10.5539/mas.v6n3p22

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