Breeding of Marker-free Indica Three-lines Restorer with ZmC4Ppc by Marker-assisted Selection

Xunchao Xiang, Yanli zhang, Yanfang Xu, Jihang Li, Libin He, Ping Li

Abstract


Enhancing photosynthetic productivity of rice is always the key goal of researchers by to use exogenous C4 genes. The full-length of intact maize gene of C4-specific PEPCase (ZmC4Ppc) is 6781 bp that makes it difficult for marker-assisted selection (MAS) in rice. The specific marker of intact ZmC4Ppc was designed and termed as MRpc. MRpc was verified that it was a gene marker because ZmC4Ppc could be overexpressed at every developmental stage and it was used for MAS of indica restorer Shuhui881 with ZmC4Ppc and FPM881was gotten, which was tested for genetic background, PEPCase activity, net photosynthetic rate (Pn), general combining ability (GCA) and specific combining ability (SCA). The results indicated that FPM881, which similarity of genetic background had arrived at above 95%, showed a higher PEPCase activity and net photosynthetic rate than those of control. Analysis of yield components showed that some of the progenies carrying ZmC4Ppc had better GCA and SCA than those of the control. Then, six lines of FPM881 were planted in larger population by selfing and gotten selectable-marker free plants with target gene using MRpc and hpt primers. Our results indicated that introduction of ZmC4Ppc via MAS and its stable expression could increase grain yield of hybrid rice.

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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