Various Hormonal Supplementations Activate Sugarcane Regeneration In-Vitro

Ghulam Zahra Jahangir, Idrees Ahmad Nasir, Riaz Ahmad Sial, Muhammad Aslam Javid, Tayyab Husnain

Abstract


Influence of different hormonal concentrations in plant growth medium on the onset of callus and somatic embryoid induction chased by plantlet regeneration and multiplication of regenerated shoots was the main goal of these studies. Results concluded that MS medium supplemented with auxin alone (3 to 4mg/l 2, 4-D) induces callus formation (3mg/l 2, 4-D alone produced embryogenic calli) and auxin-cytokinin combination like 2, 4-D and IAA (both in 1mg/l and 2mg/l concentration) with BAP (1mg/l) found very effective for somatic embryoid induction. Plantlet regeneration from embryogenic calli, as somatic embryogenesis, found good in auxin-cytokinin combination of 2, 4-D and IAA with BAP but in different concentrations i.e. 2, 4-D and IAA 2mg/l with 2 and 3mg/l BAP.

 


Full Text: PDF DOI: 10.5539/jas.v2n4p231

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.