Cloning of 3’ End cDNA of Ascorbate Peroxidase Gene from Fragaria × ananassa cv. Toyonaka

Yanxia Hou, Haoru Tang, Yong Zhang, Qing Chen

Abstract


A fragment of 3’ end cDNA of apx in Fragaria × ananassa cv. Toyonaka was cloned by rapid amplification of
cDNA 3’ end (3’RACE) PCR. By joining the sequence with the known fragment sequence which we had
submitted to GenBank (FJ896040), a 3’-end partial cDNA of 672bp was obtained, encoding 140 amino acids.
The sequence contained the corresponding coding sequence of 424bp long including a stop codon TAA, and the
3’-untranslated region(3’UTR) of 248bp including a potential polyadenylation signal (AAATAA) and poly(A) of
28bp long. Homology analysis of nucleotide sequence and deduced amino acid sequence on the apx gene
showed that it shared high homology with different plants species, among which it shared the highest sequence
homology with Fragaria × ananassa cv. Yoho.


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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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