The Deviation on the Determination of Microbial Biomass Carbon in Biochar Amendment Soil with Fumigation Extraction

Hongguang Cheng, Xinqing Lee, Like Zhang, Bin Fang, Fang Yang


Fumigation extraction (FE) is a well proven method to determine microbial biomass carbon. However, the underestimation is generated when FE method was used to determine the microbial biomass carbon in biochar amendment soil. In this study, the microbial biomass carbon in biochar amendment soil which was added glucose or ultra-pure water was measured with FE. The data showed the dissolved carbon which was adsorbed by the soil was increased with along the amount of added biochar. This result indicated that little effect was present on the determination of microbial biomass carbon in biochar amendment soil with FE. Under the rough assessment, the underestimation of 3% and 5% were generated when the amount of biochar was 5% and 10% respectively.

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Journal of Agricultural Science   ISSN 1916-9752 (Print)   ISSN 1916-9760 (Online)  E-mail:

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