PCR-RFLP Analysis of cpDNA in Tea Cultivars (Camellia sinensis L.) in Sichuan of China

Shengxiang Chen, Guinian Qi, Huan Li, Yao Zou, Hongli Shan


The genetic diversity among 30 tea cultivars in Sichuan of China was investigated by PCR-RFLP analysis of cp DNA. 7 sets of chloroplast primers could produce one or more than one distinct bands by direct electrophoresis in 2% agrose gels. After the amplified  products were digested by 10 restriction enzymes, a total of 135 bands were detected, among which 98 bands (72.59%) were polymorphic. The cpDNA PCR-RFLP based genetic distance (GD) among 30 tea accessions ranged from 0 to 0.071, with the mean of 0.049.

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DOI: https://doi.org/10.5539/jas.v4n5p25

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Journal of Agricultural Science   ISSN 1916-9752 (Print)   ISSN 1916-9760 (Online)  E-mail: jas@ccsenet.org

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