Novel BsuRI-c.930A>G-FSHb Associated with Litter Size Traits on Large White X Landrace Crossbred Sows

Nakarin Pripwai, Supamit Mekchay

Abstract


The objective of this experiment was to identify the novel single nucleotide polymorphisms (SNPs) on porcine follicle stimulating hormone b subunit (FSHb) genes. Moreover, their association with litter size traits in commercial pigs will be analyzed. 1,155 Large White x Landrace crossbred sows were bled and DNA was extracted. The records included total number of piglet born (TNB), number of piglet born alive (NBA), number of piglets stillbirth (SB) and number of piglets mummified (MM). The known sequence of porcine FSHb (GenBank accession no. D00621.1) was screened homology to known sequences in term of express sequenced tags (ESTs) in public domain, GenBank. The primers were designed for amplified the novel BsuRI-c.930A>G-FSHb fragment which confirmed by PCR-RFLP and nucleotide sequencing then genotyping.  The favorable cut homozygous G/G allele was highly significant higher than A/G allele in terms of TNB and NBA. While, published marker HaeIII-g.5894A>G-FSHb was not significant difference in any litter traits. For haplotype analysis, c.930A>G-g.5894A>G of FSH?, unfavorable GG/AA haplotype was significantly lower TNB and NBA than other haplotypes. Conversely, this haplotype was significantly higher MM than others. The study concluded that BsuRI-c.930A>G and the haplotype of HaeIII-g.5894A>G - BsuRI-c.930A>G of FSHb may used for marker-assisted selection on pig breeding program.


Full Text: PDF DOI: 10.5539/jas.v4n1p104

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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