Bioassay-Guided Isolation, Purification and Charaterization of Antimicrobial Compound From Acidic Metabolite of Piper umbellatum Seed Extract

A. E. Ejele, C. I. Akalezi, I. C. Iwu, L. N. Ukiwe, C. K. Enenebaku, S. U. Ngwu


The antimicrobial potential of ethanol extract of Piper umbellatum seeds and its acidic, basic and neutral metabolites were evaluated using the disc diffusion method. The results showed that the acidic and neutral metabolites exhibited greater antibiotic activities against the tested microorganisms; Coliform bacilli, Salmonella typhi and Staphylococcus aureus and gave inhibition zone diameters greater than 28 mm against both C. bacilli and S. aureus. These results were interpreted in terms of differences in phytochemical composition of these metabolites and indicated that an effective anti-Staphylococcal drug could be developed from P. umbellatum extract for chemotherapy of diseases caused by S. aureus, which is known all over the world to develop resistance to most potent antibiotic drugs. Chromatographic purification of the acidic metabolite gave five fractions whose antimicrobial potential against some pathogens was evaluated and compared with Amoxil (a positive standard antimicrobial drug). The results showed that all the chromatographic fractions possessed inhibitory activities against the tested microbes. Spectroscopic analysis of IR, 1H– and 13C–NMR data suggested that the active principle in acidic metabolite of P. umbellatum could be Naringin, a naturally occurring polyphenol and antibiotic drug.

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International Journal of Chemistry   ISSN 1916-9698 (Print)   ISSN 1916-9701 (Online)

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