Overexpression of the CBF2 transcriptional Activator Enhances Oxidative Stress Tolerance in Arabidopsis Plants

Ron Porat, Michal Sharabi-Schwager, Alon Samach


The C-repeat/dehydration-responsive-element binding factor genes (CBF1-3) are transcriptional activators
involved in governing plant responses to low temperatures; their overexpression enhances plant frost tolerance.
We found that overexpression of CBF2 in Arabidopsis enhanced oxidative stress tolerance as compared with
wild-type plants, an effect that was manifested in: increased seed germination rates on Petri dishes containing
H2O2; delayed leaf senescence following incubation with H2O2; and delayed wilting and senescence after
spraying whole plants with paraquat, a generator of superoxide radicals. Transcript profiling analysis using the
Affymetrix ATH1 genome array revealed that overexpression of CBF2 did not affect expression of reactive
oxygen-scavenging genes but rather, remarkably enhanced expression of oxidative-stress-responsive
transcription factor genes. Overall, the present findings suggest that overexpression of CBF2 in Arabidopsis
enhances oxidative stress tolerance, most likely via activation of a network interaction among stress-related
transcription-factor genes.

Full Text:


DOI: http://dx.doi.org/10.5539/ijb.v3n2p94

International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.


scholar_logo_lg_2011_120 proquest_logo_120 lockss_logo_2_120