Characterization of Human Adipose Tissue Derived Hematopoietic Stem Cell, Mesenchymal Stem Cell and Side Population Cells

dhanasekaran marappagoundar, indumathi somasundaram, Anand kumar Arachimani, Rajkumar sankaran, Pachaiyappan viswanathan, Sangeeth kumar, Vidya Vipparala, Baskaran Mayakesavan

Abstract


Adipose tissue represents an abundant and accessible source of multipotent adult stem cells, which appear to possess a
yet-undetermined degree of plasticity. Subcutaneous adipose tissue is much studied in recent years than omentum fat
tissue. Hence the objective of this work is to characterize the freshly isolated human adipose tissue derived stromal
vascular fraction cell relative to passage 0 from subcutaneous fat and the omentum fat. The stromal vascular fraction of
both subcutaneous and omentum fat contains hematopoietic and mesenchymal stem cell population where as in P0 the
mesenchymal stem cell alone was retained. The side population ABCG2 is present in subcutaneous fat, whereas no
ABCG2 expression is found in omentum fat. This study showed that the omentum fat exhibit higher percentage of
hematopoietic cells compared to subcutaneous fat in both SVF and P0. It is concluded from this study that not only
subcutaneous fat, but also omentum fat derived stem cells serve as a therapeutic potential in Regenerative medicine.


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International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

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