An Effective Vaccine Strategy of Translating Apoptotic Tumor Cells into Cancer Vaccine

Ning Yang, Anjun Liu

Abstract


Cancer vaccine has been regarded as the most attractive treatment for the patients. Our previous work showed that cartilage polysaccharide of short-chain (CPS) could induce apoptosis of L1210 cell lines through mitochondria apoptosis pathway and mice treated with CPS didn’t develop tumors after L1210 cell inoculation when compared to control mice without treatment. In present work, we developed a vaccine based on previous experiments and results showed that four in ten mice vaccinated didn’t show proliferation of ascite tumors while the mice in the control group didn’t show inhibition of tumor growth. We further identified the antibody with immunoflourescence and western blot from the serum of immunized mice which can recognize a protein with molecular weight of 35 kDa. Finally, we used mass spectrometry and identified the target recognized by the antiserum annexin A2, which is involved in cell proliferation, differentiation and apoptosis processes and is tightly associated with cancer progression and chemoresistance.


Full Text: PDF DOI: 10.5539/cco.v1n1p167

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Cancer and Clinical Oncology ISSN 1927-4858(Print) ISSN 1927-4866(Online)

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