Study on Extraction and Purification Process of Capsicum Red Pigment

Dan Chen, Zanmin Wu

Abstract


Capsicum red pigment extracted from the dry pepper is a kind of high-quality natural dye which has anticancer and
cosmetic properties. First, Qiu North spicy was selected as the experimental objects by comparising the peel meal rate
and relative amount of pigment of Fructus capsici, Qian chilli 2, and Qiu North spicy. Then, before extracting paprika
dye, sodium hydroxide was used to eliminate the piquancy. Capsicum red pigment was extracted in Soxhlet extractor
with 95% ethanol. The elution condition of column chromatographic separation was obtained by thin-layer
chromatography, and the purity of capsicum red pigment was identified by methods of infrared spectrum(IR). Finally,
the color value of capsicum red pigment was determined by spectrophotometer with 460nm. Results showed that the
optimal process: Concentration of peppery removal agent (sodium hydroxide) was 10%, the holding temperature was
80????, solid-liquid ratio was 1:20 (g/ml), the extraction time was 120min and the times of extraction were twice. Eluent
of silica gel column chromatography separation consisted of petroleum ether and 90% ethanol with the volume ratio of
2:1. The color value of capsicum red pigment (E1cm
1%460nm) could reach to 125 and 2.88% of yield could be gained.
The process was good to extract and purify capsicum red dye whose stability was high at neutral and weak acid
solution.

Full Text: PDF DOI: 10.5539/jas.v1n2p94

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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