Influence of Marker Genes on Physicochemical Properties of Starch Produced by Transgenic Cassava (Manihot esculenta Crantz) Plants

Jelili T. Opabode, John A. Akinkunmi, Oluyemisi A. Akinyemiju

Abstract


The study monitored the influence of genetic modification of cassava plants with NPT-II and GUS genes on starch content and physicochemical properties of transgenic starch for three cycles of vegetative propagation from 2010-2012. There were no significant (p>0.05) differences in the starch content and physicochemical properties among the three cycles of vegetative propagation. The NPT-II and GUS genes were strongly expressed in the vascular bundle, starch synthesizing and storage cells. The type of TME 12 variety (transgenic vs non-transgenic) has significant (p<0.05) influence on starch content, crude fibre, pasting viscosity and breakdown value. Starch content and crude fibre of non-transgenic plant (83.9%, 3.6%) were significantly (p<0.05) higher than that of transgenic plant (77.5%, 2.9%). Similarly, pasting viscosity and breakdown value (783.2 mPa s, 284.2 mPa s)  of non-transgenic  plants were greater than that of transgenic plant (621.0 mPa s, 133.1 mPa s) significantly. The implications of the findings on future genetic modification of cassava with NPT-II and GUS genes for starch quality improvement are discussed.


Full Text: PDF DOI: 10.5539/jas.v5n1p201

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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