The FaRE1 LTR-retrotransposon Based SSAP Markers Reveal Genetic Polymorphism of Strawberry (Fragaria x ananassa) Cultivars

Nataliya V. Melnikova, Anna V. Kudryavtseva, Anna S. Speranskaya, Anastasiya A. Krinitsina, Alexey A. Dmitriev, Maxim S. Belenikin, Vladimir P. Upelniek, Elena R. Batrak, Irina S. Kovaleva, Alexander M. Kudryavtsev

Abstract


The genetic diversity of strawberry F. x ananassa varieties with respect to FaRE1 LTR retrotransposon integration sites was studied for the first time. The SSAP method with a single combination of primers revealed 35 polymorphic sites of FaRE1 LTR retrotransposon integration in the strawberry genome. Most of the studied varieties (36 out of 46) had unique combinations of polymorphic FaRE1 insertions but 5 pairs of varieties did not differ in their SSAP markers. Assessment of genetic similarity of strawberry varieties using SSAP markers did not reveal distinct genetic clusters, which may be explained by a relatively short history of selection of this cultivar and by crossing within the same genetic pool.


Full Text: PDF DOI: 10.5539/jas.v4n11p111

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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