The Anti-brain Ageing Effects of Krill Phosphatidylserine in SAMP10 Mice

Jun Wang, Hongtao Lei, Pengtao Li, Li Han, Jincai Hou, Yangyang Yan, Haiping Zhao, Tomoko Tsuji

Abstract


We examined the biological effect of krill phosphatidylserine (K-PS) on brain ageing and investigated the mechanisms that how K-PS works on the brain using senescence-accelerated mouse (SAM) model with age-associated neurodegeneration. SAMP10 mice with 5 months of age were treated orally once a day for 75 days with 10 or 100 mg kg?1 d?1 K-PS (low and high dose). The effect of K-PS treatment on the cross-sectional area and Nissl body number of the neocortex at point C, an area prone to atrophy in SAMP10 mice, behavior and oxidative stress were evaluated by Image-Pro Plus software, step-down testing, and malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) assays. Ionized calcium binding adaptor molecule 1 (Iba-1) and insulin-like growth factor 1 (IGF-1) expression was evaluated using immunohistochemistry. Low or high doses of K-PS significantly increased the cross-sectional area and Nissl body number at point C, partly reversed memory impairment, increased the activity of GSH-Px and reduced MDA levels. Moreover, immunohistochemistry indicated that K-PS suppressed Iba-1 expression and upregulate the expression of IGF-1. These findings suggest that K-PS could prevent or slow the progression of brain atrophy and neuronal damage associated with aging by the inhibition of Iba-1 and the upregulation of IGF-1 expression.


Full Text: PDF DOI: 10.5539/jas.v4n9p196

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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