Micropropagation of GF 677 Rootstock

Rahim Nazary Moghaddam Aghaye, Abbas Yadollahi


The aim of this investigation was to study micropropagation of Almond GF rootstock on in vitro. Explants were cultured in MS medium containing 5 concentrations of 6- Benzylaminoporine (BAP) 0.1, 0.6, 1, 1.5 and 2 mg 1-1 along with control treatment. Each treatment includes 5 replications. After 5 weeks of culture, the results showed that the number and length of shoots in treatment containing 1 mg 1-1 BAP was more than the other treatments and control. The number of shoot that was obtained in treatment of 1 mg 1-1 BAP was 2.98 (Table 1). The lowest number of shoot was 0.4 due to control treatment. For rooting, explants with 3-5 cm length were transferred to different rooting treatments (Table 2). The results revealed that the best rooting was obtained with treatment 3 mg 1-1 IBA. Number and length of roots were 2.167 and 11.53, respectively. Also results showed that putting explants in dark for 1 week is more effective than light.

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DOI: https://doi.org/10.5539/jas.v4n5p131

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Journal of Agricultural Science   ISSN 1916-9752 (Print)   ISSN 1916-9760 (Online)  E-mail: jas@ccsenet.org

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