Effects of Myo-Inositol and Thiamine on Micropropagation of GF677 (Peach × Almond Hybrid)

Sadegh Sepahvand, Ali Ebadi, Kazem Kamali, Saeid Ali Ghaemmaghami


GF677 is a vigorous rootstock, used for both peach and almond worldwide. Nowadays, it is propagated is mainly through micro propagation. Widening and undesirable growth of leaves as well as poor rooting are major problems during its in vitro culture. In this study, effects of myo-inositol at 10 and 100 mg/l In MS medium were studied at explants establishment and shoot growth stages. Thiamine was also used in LS medium at 0, 1.6, 2.8 and 4 mg/l at rooting stage. This experiment was conducted in Randomized Complete Design with 10 replications. Myo-inositol at 10mg/l could prevent undesirable widening of leaves as well as rosetting of shoots. Thiamine at 1.6 mg/l was highly effective in rooting of shoots.

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DOI: https://doi.org/10.5539/jas.v4n2p275

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