Preparation of Low Molecular Weight Chitosan by Complex Enzymes Hydrolysis

Huafei Xie, Zhengyu Jia, Jinhui Huang, Cuirong Zhang

Abstract


Through the determination of viscosity average molecular weight, enzyme activity, hydrolysis ratio and reducing sugar concentration, the optimal technological conditions of complex enzymes composed of commercial ?-amylase, cellulase and pectinase were investigated. Results indicated that complex enzymes could result in low molecular weight chitosan ranged from 1000 to 4000 after enzymatic degradation for 2 h under the condition of zymolyte ratio 1:5(m/m), pH 5.3 and temperature 56?, and the structure of those products failed to vary obviously by FTIR analysis.


Full Text: PDF DOI: 10.5539/ijc.v3n2p180

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

International Journal of Chemistry   ISSN 1916-9698 (Print)   ISSN 1916-9701 (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.