Extracellular Lipase Enzyme Production by Seed-Borne Fungi under the Influence of Physical Factors

Rajendra Balasaheb Kakde, Ashok Mangesh Chavan

Abstract


Present paper deals with study of impact of physical factors on extracellular lipase activity of some dominant oilseed-borne fungi. In present investigation, seed-borne fungi were isolated from abnormal oilseeds by agar plate method. Of the 11 genera and 17 species of fungi isolated, 10 dominant fungi were selected to study their lipase enzyme activity. Various parameters such as temperature, pH, light and incubation period were studied in order to determine the optimum conditions for lipase production of ten dominant fungi. The lipase present in the broth was assayed by cup-plate method. It is revealed from the result that lipase activity of storage fungi was found to be optimum at temperature 200C, pH 6.5, incubation period of 20 days and continuous light.

 


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International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

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