A Rapid and Simple Method for Brassica Napus Floral-Dip Transformation and Selection of Transgenic Plantlets

Juan Li, Xiaoli Tan, Fuge Zhu, Jingjing Guo


The floral-dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium was applied
in the Brassica napus transformation, but it involves a number of relatively time-consuming and laborious steps,
including manipulating an Agrobacterium tumefaciens culture and aseptic procedures for the selection of plant lines
harboring antibiotic-selection markers. It calls for a long time to prepare the buffered media. To circumvent these
bottlenecks, we have developed a rapid and simple method. We find that Brassica napus can be transformed by dipping
directly into an Agrobacterium tumefaciens culture supplemented with surfactant, eliminating the need for media
exchange to a buffered solution. We report a method of transformant selection by soaking seeds with antibiotic. These
methods save time and money, and reduce the possibility of contamination.

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DOI: https://doi.org/10.5539/ijb.v2n1p127

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