A Rapid and Simple Method for Brassica Napus Floral-Dip Transformation and Selection of Transgenic Plantlets

Juan Li, Xiaoli Tan, Fuge Zhu, Jingjing Guo

Abstract


The floral-dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium was applied
in the Brassica napus transformation, but it involves a number of relatively time-consuming and laborious steps,
including manipulating an Agrobacterium tumefaciens culture and aseptic procedures for the selection of plant lines
harboring antibiotic-selection markers. It calls for a long time to prepare the buffered media. To circumvent these
bottlenecks, we have developed a rapid and simple method. We find that Brassica napus can be transformed by dipping
directly into an Agrobacterium tumefaciens culture supplemented with surfactant, eliminating the need for media
exchange to a buffered solution. We report a method of transformant selection by soaking seeds with antibiotic. These
methods save time and money, and reduce the possibility of contamination.

Full Text: PDF

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.